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Protesters carried Palestinian flags and signs while pounding drums and chanting, according to footage circulating social media.
AOC made the announcement via social media less than 12 hours ahead of the address.
The DNC begins Monday in Chicago.
Mediators are to meet again this week in Cairo to try to cement a cease-fire.
U.S. Secretary of State Antony Blinken headed to the region to try to seal a cease-fire deal after months of negotiations.
Of thousands of delegates gathering at the DNC, just 36 belong to the "uncommitted" movement sparked by dissatisfaction with Biden's handling of the Gaza war.
The new push for an end to the Israel-Hamas war came as the Palestinian death toll in Gaza climbed past 40,000.
A federal judge ruled this week UCLA cannot allow protests on campus that block Jewish students' access to the university.